产品货号：
GS4496
中文名称：
重组人TNF-α(带His标签)
英文名称：
Recombinant Human Tumor Necrosis Factor-alpha, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 18.3kDa,a single,non-glycosylated polypeptide chain containing 164 amino acids with Met and 6×His at N-terminus.
储存条件：冷冻（-20℃）
概述
Tumor necrosis factor alpha(TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.The biologically active native form of TNF-α is reportedly a trimer.Human and murine TNF-α show approximately 79%homology at the amino acid level and cross-reactivity between the two species.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人TNF-α(带His标签)，TNF-α, His-tagged, Human
储存条件：冷冻（-20℃）
概述
Tumor necrosis factor alpha(TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.The biologically active native form of TNF-α is reportedly a trimer.Human and murine TNF-α show approximately 79%homology at the amino acid level and cross-reactivity between the two species.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml,corresponding to a specific activity of>2.0×107 IU/mg in the presence of actinomycin D. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.0. |
| 内毒素 | Less than 1 EU/μg of rHuTNF-α,His as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL |
相关搜索：重组人TNF-α(带His标签)，TNF-α, His-tagged, Human